The Cold War bomber, which is based at Doncaster’s Robin Hood Airport, took off at 2.45pm and made a short 15 minute flight around the vicinity of the airport.
Operated by charity Vulcan to the Sky Trust, Vulcan XH558 is one of the world’s most popular aircraft.
XH558 joined Britain’s V-Force in 1960 and served in a wide range of roles until 1984, followed by a period in the RAF’s Vulcan Display Flight until being sold to a private collector in 1993.
Trustee Richard Clarke said: “Vulcan is an icon and very important in our aviation history.”
The bomber loses its flying permit at the end of the month but trustees are planning to open a visitor centre with Vulcan forming the centrepiece.
Mr Clarke added: ”It’s sad it has got to stop flying but there is a new life for the Vulcan.”