The 22-year-old’s loan spell at the Keepmoat Stadium ended prematurely last month after he suffered a broken foot.
Rovers boss Darren Ferguson, who wants to sign Sheffield United midfielder Ben Whiteman on a permanent deal, will not be pursuing the Norwich City defender.
“No, he [Toffolo] won’t be coming back,” Ferguson told The Star.
“At the moment I’ve only really got scope to bring in Ben and that’s what I’m fully concentrating on, especially now it’s a permanent.
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“That’s all I’ll be able to bring in over the course of the window.”
He added: “Looking at the squad I’ve got at the moment there’s probably one or two I might have been prepared to let out on loan but that’s not the case now because I haven’t got the numbers.
“What we had on Saturday is what I’ve got.
“I can’t be fully one hundred per cent that [Luke] McCullough and [Alex] Kiwomya will come back like they were when then stopped playing.
“So I haven’t even added those two to the squad at the moment.”
Rovers are expected to complete a deal for 18-year-old Finn Harps defender Shane Blaney next week following a successful trial.