Public Health England figures show that 23,433 people had been confirmed as testing positive for Covid-19 by 9am on Tuesday, April 20, in Doncaster up from 23,408 the same time on Monday.
The health body recently changed the way it records the figures.
Cases identified through a positive lateral flow test – those which can deliver results within half an hour – will no longer be counted if the person then takes a PCR test, which is sent to a lab to be checked, and receives a negative result within three days.
Across the UK, the number of recorded cases increased by 2,524 over the period, to 4,393,307.
Doncaster's cases were among the 390,006 recorded across Yorkshire and The Humber, a figure which rose by 307 over the period.
Cumulative case counts include patients who are currently unwell, have recovered and those that have died.