Steve Boden’s side, who have started the new season with two wins and two defeats, travel to Dillingham Park after two blank weekends.
They sit second bottom in the 11-team table but only seven points behind early leaders Ealing Trailfinders.
Last time out Knights beat Jersey Reds 27-24 at Castle Park and they have also defeated bottom team London Scottish on home soil.
But they are yet to pick up a point on the road after defeats at Coventry and Bedford Blues.
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Although Ampthill are not fully professional they aspire to be the most professional community rugby club in the country. It is an old-fashioned style club with a seemingly endless trek through the woods to get to their pitch from their clubhouse.
Fly-half Russell Bennett is the equal-second best kicker in the Championship, just a point from the top. Knights can’t give away penalties because they are likely to be punished.
Ampthill started their season with an away defeat to Nottingham before securing a narrow home win against Coventry. Like Knights they have beaten London Scottish and lost to Bedford, while they go into this weekend’s game on the back of a 17-17 draw at Jersey.
Knights won 19-17 at Ampthill in last season’s shortened competition. However, Ampthill prevailed 30-26 in the corresponding fixture in 2019/20, Ampthill’s first season in the Championship.