Newly-crowned Doncaster Sunday Alliance League Premier Division champions Canal Tavern lost for only the second time in the league this season.
The Thorne side, who were playing their last game of the season, lost 4-2 away to fifth-placed Athletico Tap Maker for whom Ash Hunter notched a hat trick and Robert Allan also chipped in with a single strike.
Defending champions Sprotbrough face a battle to claim the runners-up spot after suffering a 2-1 defeat at home to ninth-placed Leger Lions.
Fouth-placed FC Glasshouse also suffered a surprise home defeat, fourth-bottom Stainforth beating them by the odd goal in seven.
Graceholme, one of the division's form sides in recent weeks, had to settle for a share of the spoils when drawing 3-3 with visiting Rhino's (Gareth Collinson 2, Danny Porteous).
Fellow strugglers Wheatsheaf (Dan McNiel) and Toll Bar, who were playing their last game of the season, drew 2-2.
Championshp leaders Rossington United saw their lead cut to just one point after being held to a 1-1 draw by third-bottom Rosehill Press.
Bullcroft Sports & Social, who have a game in hand and an identical goal difference, received a walkover against third-placed Lonsdale.
Division One leaders Eve's Trust Dynamo's must now rely on other results going their way in the run-in after suffering a shock 5-3 defeat away to lowly Finningley Athletic.
The Askern, who were without a game, remain three points adrift of the leaders and C & R Sports - who have completed their fixtures - but now have two games in hand.
Arksey Victoria beat FC Gatehouse by the odd goal in seven in the only other League One clash of the day.
It continues to be a two-way battle for the Division Two title between leaders Legion Park Rangers and third-placed Rycroft Rovers.
Rangers beat Highwayman 7-2 to remain seven points clear of Rovers having played three more games. Ryan Clegg 2, Mark Ridley, Jamie Murray, Scott Henderson, Marv Stacey and Nigel Wilson shared the Rangers' goals with Glenn Norton and Billy Worton replying for the home side.
Rovers, meanwhile, beat FC Wadworth 5-0.
Mid-table FC Cumberland beat bottom team Pit Club 3-2.
Favourites Balby Athletic, who started the day in third place six points behind leaders Fairway Athletic with three games in hand, slumped to a 5-1 Division Three defeat at home to fourth-placed Balby Academy of Sport Inter for whom Richard Rodgers 3, Brad Parks and James Kenny netted.
Fairway took full advantage when beating White Hart (Askern) 4-0. Windmill Wanderers (Gary Hawker 2, Gareth Stent) beat bottom-four rivals Newlands (Sam Spink, own goal) 3-2.