Two unnamed players have tested positive for the virus while several others are displaying symptoms.
And with no time to test the rest of the squad and coaching staff ahead of the Accrington game at the Keepmoat, the club has been forced to postpone the game.
It is understood the rest of the squad will be tested over the next couple of days to determine whether the virus has spread throughout the group.
Tuesday’s trip to Fleetwood Town is now in serious doubt, while the trips to Peterborough United and Oxford United are also under threat, should Covid-19 be more prevalent within the squad.
A statement from the club read: “Doncaster Rovers can confirm that two unnamed players have tested positive for COVID-19 with more players displaying symptoms.
“The safety and health of our players, staff and everyone around the club is paramount and therefore we have informed the EFL that we are unable to fulfil our fixture against Accrington Stanley.
Since returning to training in August 2020, Doncaster Rovers have implemented and followed wide-ranging and comprehensive protocols to protect its players and staff adhering to all of the latest advice and guidance provided by the Government and the EFL.
“The club will not be naming individuals who are currently self-isolating and asks that their privacy is respected.
“We would like to thank Accrington Stanley for their understanding following news of the postponement.”
League One has been badly affected by the virus, with Rovers’ game the fifth on Boxing Day to be postponed.