The protest, organised by Road Blockage N-Lincs, aims to cause disruption on the M180 on Monday.
The rolling road block will start at 6am from the premises of Merc Trans on Normanby Road, Scunthorpe.
A spokesman said: “Who is with us on the 4th July?
“If you would like to come to our yard for 5:30am please do. It will be nice to see everyone joining in.”
Organisers say that ‘all vehicles are welcome’ to join the early morning protest.
It comes as fuel prices spiral because of the war in Ukraine.