The mounted police section which patrols the Isle of Axholme has been disbanded in a cost saving measure.
Humberside Police’s four horses were sold for £40,000 to two police forces and a private buyer last month.
More than 2,000 people had signed a petition calling for the force to keep its horses.
Matthew Grove, police and crime commissioner for Humberside, said removing the unit should save the force £500,000 a year.
He has previously said the mounted section was “only used 27 times” in the last full year and the closure would generate £2m savings over four years to maintain frontline services elsewhere.
There are now plans for a volunteer-led mounted section for rural patrols.
Horses will be hired from other police forces if they are needed to patrol events, such as football matches.
Two horses, Harvey and Captain, were sold to Greater Manchester Police for a total of £21,000, while George was sold to South Wales Police for £14,050.
Another horse, Lord George, was sold to a private buyer for £5,000 because he was “no longer suitable for police work”, Mr Grove’s spokesman Paul Cheeseman said.
Mr Cheeseman added that three PCs and a sergeant, who worked in the mounted unit, have been “redeployed elsewhere in the force.”